Diego Costa could make a second international debut against Italy when Spain host Cesare Prandelli's men in Madrid on Wednesday.
Having previously come on as a substitute in two friendlies for Brazil last March - the first against Italy - Costa received Spanish nationality and made the controversial decision to switch allegiances.
The Atletico Madrid striker, who boasts 21 La Liga goals this season, is now in line to represent Spain for the first time, having been included in the defending world champions' squad to face Italy.
Coach Vicente del Bosque said of Costa: "He's an unusual case, because he had never been called up before and because he was born in Brazil.
"But he's a player who has been called up on the back of what he has achieved with his club and because we believe that he can help us. That's why we've called him up; for no other reason."
Italy can be expected to pose a stern test of Spain's FIFA World Cup credentials and the two sides are familiar with each other, having met four times inside the last three years.
Spain's only defeat during that time came in a friendly in August 2011.
Del Bosque's men then drew with Italy in Group C of the UEFA European Championships a year later before running out 4-0 winners against the same opponents in the final of the competition.
Spain needed penalties to see off Italy again in the FIFA Confederations Cup semi-finals last year following a goalless draw.
With Del Bosque set to name his squad for the 2014 World Cup on May 25 - four days ahead of a friendly with Bolivia - this week's game against Italy could represent the last chance for Spain's fringe players to nail down a place.
Italy, meanwhile, have drawn both of their friendlies - against Germany and Nigeria - since qualifying for the finals and have fixtures with the Republic of Ireland and Luxembourg pencilled in before a World Cup opener against England in Manaus.
Roma midfielder Danielle De Rossi has been left out by national coach Prandelli after throwing a punch at Mauro Icardi during Saturday's goalless draw with Inter.
Striker Mario Balotelli is also absent with a shoulder problem, but uncapped Torino striker Ciro Immobile could feature after scoring 13 Serie A goals this season.
Both sides were unbeaten in qualifying and topped their respective groups, although Spain's last two outings have been less than convincing.
Spain narrowly beat Equitorial Guinea 2-1 in November before going down 1-0 to South Africa three days later.
|Coach: Del Bosque|
for Fàbregas 46'
for Casillas 46'
for Busquets 46'
for Sergio Ramos 66'
for Iniesta 66'
for Pedro 82'
for A. Candreva 46'
for C. Maggio 46'
for D. Criscito 46'
for Thiago Motta 62'
for A. Cerci 69'
for P. Osvaldo 69'
|Allegri expects Juve to progress|
|Onuoha in race to face Villa|
|Ronaldo baffled by El Clasico scheduling|
|Zaluska fine for Astra clash - Deila|
|Pochettino sets sights on trophy within two years|
|J.League Preview: Gamba not giving up|
|Lambert: Cole starting to make an impact|
|CONCACAF Champions League Review: Pachuca miss out on top seeding|
|Peruvian Primera Division Review: Clasico triumph sends Alianza top|
|Real Salt Lake 2 Chivas USA 0: RSL leapfrog Dallas|
|Copa Sudamericana Review: River, Estudiantes to meet in quarters|
|Brazilian Serie A Review: Corinthians rise in the standings|
|Ecuadorian Primera A Review: Independiente del Valle, Emelec win again|
|Marcelinho: ´Why can´t we go through?´|
|Sousa ´proud´ despite defeat|
|Galatasaray brush off continental defeat|
|Copa Argentina Review: Atletico Rafaela complete last four|
|Olympiacos president thrilled with ´flawless´ win|
|Anderlecht deserved to win - Hasi|
|Villas-Boas eyes Zenit Champions League riposte|
|Koke wants more after starring in Atletico rout|
|Ancelotti pleased to see Real hit top gear|
|Bender pleased with Leverkusen effort|
|Benfica deserved to win, says Jesus|
|Rodgers will ´deal with´ shirt-swapping Balotelli|
|Jardim disappointed with Monaco attack|
|Tevez calls for mental change at Juventus|
|´Proud´ Ronaldo not focusing on Raul record|
|Wenger hails Arsenal spirit|
|Klopp salutes quick start in Dortmund rout|
|Ludogorets 1 Basel 0: Bulgarians secure historic triumph|
|Bayer Leverkusen 2 Zenit 0: Russians´ streak ends at 16|
|Anderlecht 1 Arsenal 2: Gibbs, Podolski score late for visitors|
|Olympiacos 1 Juventus 0: Roberto heroics help win it for hosts|
|Atletico Madrid 5 Malmo 0: Koke stars as Simeone´s men ease to win|
|Monaco 0 Benfica 0: 10-man visitors claim dour draw|
|Liverpool 0 Real Madrid 3: Ronaldo scores again as European champions stroll on Merseyside|
|Galatasaray 0 Borussia Dortmund 4: Klopp´s men rampant in Istanbul|
|Championship Preview: Watford and Boro battle at the top|
|Platini demands CAF apology|
|Metalist 0 Legia Warsaw 1: Dazzling Duda seals victory|
|Martinez: Lille double-header like a cup final|
|Evans seeks ´second chance´ after release from prison|
|MLS Preview: Whitecaps, Timbers chase final play-off spot|
|Bellusci is no racist - Cellino|
|Thai Premier League Review: Chonburi go top, Air Force Central and Songkhla relegated|
|UEFA rule out CSKA punishment|
|J.League Review: Urawa extend lead despite draw, Tokushima relegated|
|UEFA investigating Luiz Adriano allegations|
|Bentaleb out of Asteras Tripolis clash|
|Rafael: Chelsea are beatable|
|Cannavaro assets seized in tax fraud probe|
|Leverkusen ordered to repay over €16m to former sponsor|
|Remy hopeful of facing United|
|Gerrard: I may regret Real snubs|
|FA investigating Jerome racism allegation|
|Alcaraz takes over at Levante|
|Wenger backs Martinez to take his chance|
|Benatia: Media judged me too soon|
|CAF hits out at Platini|
|Mourinho calm over striker shortage|
|Kompany fumes at presence of CSKA fans|
|CONCACAF Champions League Review: Timbers eliminated|
|Jerome complains of racial abuse|
|Lanus 1 Cerro Porteno 1: Paraguayans reach quarters|
|A-League Preview: Another blow for Melbourne City ahead of derby|
|Luiz Adriano alleges racism|
|Yermakovich laments lack of quality, errors|
|Lopetegui pleased with important win|
|Luiz Adriano overjoyed with record-equalling haul|
|Blanc hails PSG´s fighting spirit|
|Melbourne City confirm Villa departure|
|Mixed feelings for Donis|
|Chelsea are ´serious contenders´ - Simundza|
|Valverde remaining positive|
|Silva slams penalty decision after Sporting loss|
|De Boer unhappy with early Ajax caution|
|League Two Review: Wycombe lose at Exeter, Burton win thriller|
|Championship Review: Derby go top, Birmingham struggles continue|
|Guardiola plays down dominant Bayern win|
|Schalke grateful for controversial penalty win|
|Rakitic takes heart from Barca performance|
|League One Review: Crewe run continues as Preston win again|
|Garcia accepts blame for Bayern mauling|
|UEFA Europa League Preview: Sevilla, Napoli hit the road|
|Drogba delight as Maribor pay the penalty|
|Schalke 4 Sporting Lisbon 3: Choupo-Moting heroics earn Di Matteo incredible win|
|BATE 0 Shakhtar Donetsk 7: Luiz Adriano the five-goal hero|
|APOEL 0 Paris Saint-Germain 1: Late Cavani strike seals victory|
|Barcelona 3 Ajax 1: Messi and Neymar help sink Dutch champions|
|Chelsea 6 Maribor 0: Drogba on target in comfortable success|
|Porto 2 Athletic Bilbao 1: Quaresma leaves it late to ensure maximum points|
|Roma 1 Bayern Munich 7: Guardiola´s men run riot in Rome|
|Slutsky delighted with CSKA spirit|
|Sloppy City frustrate Pellegrini|
|Lille v Everton: Martinez´s men look to continue recovery|
|Tottenham v Asteras Tripolis: Spurs begin big week with group leaders|
|Bale out of Clasico, confirms Ancelotti|
|Galatasaray the perfect test for Dortmund, says Klopp|
|CSKA Moscow 2 Manchester City 2: Visitors remain winless after spurning two-goal lead|
|Rodgers plays down Bale absence|
|Allegri urges Juventus to keep calm|
|Subotic wants confidence-boosting victory|
|Fernandes apologises for Taarabt-Redknapp spat|
|Lopes hopeful of return after mini-stroke|
|Gabi to miss out for Atletico|
|Inter v Saint-Etienne: Mazzarri´s men eye next step to last 32|
|Hasi warns of Arsenal threat|
|Verona hit with fine and partial stadium closure|
|Sunderland players offer fans refund after Southampton mauling|
|Schmidt wants Leverkusen reaction|
|Redknapp: Taarabt the worst professional I have ever come across|
|´I haven´t been up to my full potential´, admits Shaw|
|Flamini: Injuries no excuse for Arsenal form|
|Busquets to miss Ajax clash|
|Bale out of Anfield clash|
|Levante sack Mendilibar|
|Platini talks down Ronaldo´s Ballon d´Or chances|
|Messi and Ronaldo out of this world in UEFA Champions League space race|
|Bring on Bilbao - Lopetegui|
|J.League Preview: Petrovic positive despite Urawa form|
|Argentine Primera Division Review: Calleri inspires Boca|
|Guardiola: Garcia´s Roma reign ´wonderful´|
|Chilean Primera Division Review: Colo Colo claim key win|
|Mata had ´no relationship´ with Mourinho|
|Paraguayan Division Profesional Review: Libertad gain ground, Cerro Porteno held|
|Lovren demands better display|
|´Arrogant´ Van Gaal insists United can catch Chelsea|
|Sociedad loss a ´hammer blow´ - Arrasate|
|Genoa deserved a penalty - Gasperini|
|Passion kept me at Roma - De Rossi|
|Irvine ´bitterly disappointed´, questions Fellaini´s goal|
|Taarabt hits back at Redknapp claims|
|Genoa 1 Empoli 1: Tonelli hands visitors a point|