Diego Costa could make a second international debut against Italy when Spain host Cesare Prandelli's men in Madrid on Wednesday.
Having previously come on as a substitute in two friendlies for Brazil last March - the first against Italy - Costa received Spanish nationality and made the controversial decision to switch allegiances.
The Atletico Madrid striker, who boasts 21 La Liga goals this season, is now in line to represent Spain for the first time, having been included in the defending world champions' squad to face Italy.
Coach Vicente del Bosque said of Costa: "He's an unusual case, because he had never been called up before and because he was born in Brazil.
"But he's a player who has been called up on the back of what he has achieved with his club and because we believe that he can help us. That's why we've called him up; for no other reason."
Italy can be expected to pose a stern test of Spain's FIFA World Cup credentials and the two sides are familiar with each other, having met four times inside the last three years.
Spain's only defeat during that time came in a friendly in August 2011.
Del Bosque's men then drew with Italy in Group C of the UEFA European Championships a year later before running out 4-0 winners against the same opponents in the final of the competition.
Spain needed penalties to see off Italy again in the FIFA Confederations Cup semi-finals last year following a goalless draw.
With Del Bosque set to name his squad for the 2014 World Cup on May 25 - four days ahead of a friendly with Bolivia - this week's game against Italy could represent the last chance for Spain's fringe players to nail down a place.
Italy, meanwhile, have drawn both of their friendlies - against Germany and Nigeria - since qualifying for the finals and have fixtures with the Republic of Ireland and Luxembourg pencilled in before a World Cup opener against England in Manaus.
Roma midfielder Danielle De Rossi has been left out by national coach Prandelli after throwing a punch at Mauro Icardi during Saturday's goalless draw with Inter.
Striker Mario Balotelli is also absent with a shoulder problem, but uncapped Torino striker Ciro Immobile could feature after scoring 13 Serie A goals this season.
Both sides were unbeaten in qualifying and topped their respective groups, although Spain's last two outings have been less than convincing.
Spain narrowly beat Equitorial Guinea 2-1 in November before going down 1-0 to South Africa three days later.
|Coach: Del Bosque|
for Fàbregas 46'
for Casillas 46'
for Busquets 46'
for Sergio Ramos 66'
for Iniesta 66'
for Pedro 82'
for A. Candreva 46'
for C. Maggio 46'
for D. Criscito 46'
for T. Motta 62'
for A. Cerci 69'
for D. Osvaldo 69'
|Gabbiadini scored three times, says frustrated Puel|
|Real Madrid equal longest scoring streak in Spanish history|
|Roma are fed up - Spalletti hits back amid penalty controversy|
|Lyon´s Memphis Depay revels in two-goal performance|
|Marseille 1 Paris Saint-Germain 5: Record crowd sees OM thrashed|
|Villarreal 2 Real Madrid 3: Bale, Ronaldo and Morata complete stunning turnaround|
|Inter 1 Roma 3: Nainggolan at the double in dominant display|
|Ronaldo spot on as he takes LaLiga penalty record|
|Mourinho: Fans will camp at Ibrahimovic´s house to make sure he stays!|
|Late Barca defeat leaves Simeone with sense of deja vu|
|It is not easy to keep winning – Mourinho emotional after Manchester United success|
|Mourinho: Outstanding Ibrahimovic won us the cup|
|Pogba: That´s why Ibrahimovic came here|
|Ibrahimovic hails his predictive powers after sealing EFL Cup triumph|
|He´s incredible - Carrick hails Ibrahimovic impact after EFL Cup win|
|Mourinho equals Clough and Ferguson record with EFL Cup triumph|
|Manchester United 3 Southampton 2: Ibrahimovic settles thrilling final|
|Lewandowski focused on writing his own Bayern history after Gerd Muller comparisons|
|Muller: Goals aren´t everything for me|
|Bacca goal should not have stood – Montella|
|Kane one of the top strikers in the world - hails Pochettino|
|Messi celebrates 400th win with Barcelona|
|Atletico Madrid 1 Barcelona 2: Messi leaves it late again to stun hosts|
|Leicester should have to play without a manager, blasts Mihajlovic|
|What a first half! - Record-breaking Kane lauds Tottenham display|
|Fabregas ´humbled and proud´ after 300th Premier League game|
|Sassuolo 0 AC Milan 1: Controversial Bacca penalty proves decisive|
|Zarate confirms ACL injury|
|Bayern Munich deny Eberl approach|
|Rooney on bench for EFL Cup final|
|Tottenham 4 Stoke City 0: Kane hat-trick sends Pochettino´s side second|
|Norwich City 1 Ipswich Town 1: Knudsen and Bialkowski frustrate Neil´s men|
|´No regrets´ for Ben Yedder after rejecting PSG move|
|Mignolet: Liverpool harder to stop now than with Suarez|
|O´Neill rules out taking Leicester job and questions players´ attitude|
|´Uncle´ Toure still uncertain on Manchester City future|
|Mourinho already knows next Manchester United transfer targets|
|Newcastle Jets 1 Central Coast Mariners 1: Honours even in F3 Derby|
|Half a century of Kazuyoshi Miura: Japanese legend plays on 50th birthday|
|´Criminal´ if Shaw doesn´t succeed at United - Neville|
|Kaka scores first goal in Orlando City´s new stadium|
|Conte lauds Chelsea´s milestone man Fabregas|
|Pochettino questions readiness in Tottenham´s trophy quest|
|Mata wants to extend Manchester United contract|
|Mourinho is a winner - Cantona backs United for success|
|Allegri grateful Juve control Serie A title destiny|
|Goalless Muller was the best player, says Ancelotti after thrashing|
|Phil Neville backs Rashford to match Griezmann|
|Juventus 2 Empoli 0: Champions cruise to 10-point gap at summit|
|Howe refusing to panic as Bournemouth´s struggles continue|
|Zarate was making the difference before injury - Mazzarri|
|I owe him my eternal gratitude - Vardy´s emotional Ranieri tribute|
|Cantona: Ibrahimovic is like me|
|Watford 1 West Ham 1: Ayew halts injury-hit hosts|
|Napoli 0 Atalanta 2: Caldara double stuns Sarri´s men|
|Ancelotti: It was a perfect day|
|Championship review: Newcastle drop points, Leeds clip Owls´ wings|
|Allardyce hails Van Aanholt and Sakho|
|Koeman lays into ´lucky´ Everton´s second-half display|
|Conte: We still need 29 points for Premier League title|
|We need an Aubameyang who does not think too much - Tuchel|
|Guingamp 1 Monaco 2: Glik, Fabinho on target|
|Marco Silva blames lack of focus as Hull remain in drop zone|
|Lukaku becomes Everton´s joint leading Premier League scorer|
|Real Betis 1 Sevilla 2: Sampaoli´s men heap pressure on Real Madrid|
|RB Leipzig 3 Cologne 1: Hosts capitalise on errors to stay hot on Bayern´s heels|
|Hull City 1 Burnley 1: Keane on target as 10-man Clarets end dire away run|
|Chelsea 3 Swansea City 1: Fabianski´s error helps Premier League leaders move 11 points clear|
|Neuer reaches 100 Bundesliga clean sheets for Bayern in record-breaking fashion|
|Everton 2 Sunderland 0: Unhappy Goodison return for Moyes as Lukaku wraps it up|
|West Brom 2 Bournemouth 1: McAuley winner piles pressure on Howe|
|Crystal Palace 1 Middlesbrough 0: Van Aanholt proves difference in nervy basement battle|
|Luis Enrique dismisses claims of dressing room discontent at Barcelona|
|Bayern Munich 8 Hamburg 0: Lewandowski hits hat-trick in special day for Ancelotti|
|Freiburg 0 Borussia Dortmund 3: Aubameyang back in the goals with a brace|
|#Piegate bites back as Sutton are forced to use outfield player in goal|
|Schmeichel denies players met with Leicester board to get Ranieri sacked|
|Spalletti would not swap Dzeko for Icardi|
|Emery understands Marseille rivalry and hopes PSG can repeat Barca win|
|Ibrahimovic better than ever and could play on until he turns 40 - Mourinho|
|Simeone: Barcelona still the best team in the world|
|A-League Review: Victory stay in Sydney hunt, Roar slip up at home|
|Zidane hopes potential France return will boost Benzema|
|I have not talked to anyone at Leicester - Mancini on replacing Ranieri|
|LaLiga teams raise their game against Real Madrid, claims Zidane|
|Arrivederci, Claudio! Ranieri says his final Leicester goodbyes|
|Soldado expects Real Madrid to come out fighting after Valencia loss|
|Montella hoping to remain AC Milan coach despite impending takeover|
|Valencia hopes EFL Cup triumph can inspire late title charge|
|Pele´s son vows to clear his name after being jailed for money laundering|
|Wijnaldum highlights Kante loss as crucial to Leicester´s downfall|
|Lyon rejected chance to sign Sakho, says Aulas|
|Koeman hopes Southampton lose EFL Cup final to boost Everton´s European hopes|
|Mata insists Manchester United will not take EFL Cup final for granted|
|Simeone´s Atletico starting to decline, Abbiati claims|
|Chelsea´s Fabregas ´100 per cent´ staying at Stamford Bridge|
|´Being Joey´ - Dyche talks up Barton TV show|
|Messi the best of all time, says Jovetic|
|You can define an era - Buffon wishes Donnarumma happy birthday|
|Clement: Llorente wants to star against Chelsea|
|Koeman not interested in Everton renewal amid Barca links|
|Rivals scared of Ibrahimovic, says United team-mate Herrera|
|Schmeichel pays tribute to Ranieri|
|Yaya Toure wants to play with Neymar and Coutinho|
|Mourinho: It´s more difficult to win now|
|I didn´t sleep for six days – Conte motivated by last-gasp title loss|
|It all depends on success - Klopp knows trophies are a must|