Diego Costa could make a second international debut against Italy when Spain host Cesare Prandelli's men in Madrid on Wednesday.
Having previously come on as a substitute in two friendlies for Brazil last March - the first against Italy - Costa received Spanish nationality and made the controversial decision to switch allegiances.
The Atletico Madrid striker, who boasts 21 La Liga goals this season, is now in line to represent Spain for the first time, having been included in the defending world champions' squad to face Italy.
Coach Vicente del Bosque said of Costa: "He's an unusual case, because he had never been called up before and because he was born in Brazil.
"But he's a player who has been called up on the back of what he has achieved with his club and because we believe that he can help us. That's why we've called him up; for no other reason."
Italy can be expected to pose a stern test of Spain's FIFA World Cup credentials and the two sides are familiar with each other, having met four times inside the last three years.
Spain's only defeat during that time came in a friendly in August 2011.
Del Bosque's men then drew with Italy in Group C of the UEFA European Championships a year later before running out 4-0 winners against the same opponents in the final of the competition.
Spain needed penalties to see off Italy again in the FIFA Confederations Cup semi-finals last year following a goalless draw.
With Del Bosque set to name his squad for the 2014 World Cup on May 25 - four days ahead of a friendly with Bolivia - this week's game against Italy could represent the last chance for Spain's fringe players to nail down a place.
Italy, meanwhile, have drawn both of their friendlies - against Germany and Nigeria - since qualifying for the finals and have fixtures with the Republic of Ireland and Luxembourg pencilled in before a World Cup opener against England in Manaus.
Roma midfielder Danielle De Rossi has been left out by national coach Prandelli after throwing a punch at Mauro Icardi during Saturday's goalless draw with Inter.
Striker Mario Balotelli is also absent with a shoulder problem, but uncapped Torino striker Ciro Immobile could feature after scoring 13 Serie A goals this season.
Both sides were unbeaten in qualifying and topped their respective groups, although Spain's last two outings have been less than convincing.
Spain narrowly beat Equitorial Guinea 2-1 in November before going down 1-0 to South Africa three days later.
|Coach: Del Bosque|
for Fàbregas 46'
for Casillas 46'
for Busquets 46'
for Sergio Ramos 66'
for Iniesta 66'
for Pedro 82'
for A. Candreva 46'
for C. Maggio 46'
for D. Criscito 46'
for Thiago Motta 62'
for A. Cerci 69'
for P. Osvaldo 69'
|Russian Premier League Review: Torpedo keep survival hopes alive|
|Turkish Super Lig Review: Galatasaray crowned champions as Fener have four sent off|
|Ghana 1 Madagascar 2: Simouri nets late winner|
|Galatasaray reclaim title as Fener implode|
|Ancelotti bears no grudge after Real sacking|
|Porto complete Bueno capture|
|Lazio 1 Roma 2: Yanga-Mbiwa settles Rome derby|
|Real sack Ancelotti|
|Route to the UEFA Europa League final: Sevilla|
|Derby sack McClaren|
|Eredivisie play-offs Review: Eagles relegated, NAC Breda earn reprieve|
|Inzaghi eager for Van Ginkel stay|
|Route to the UEFA Europa League final: Dnipro|
|Dani Alves plays down Champions League favourites tag|
|Middlesbrough 0 Norwich City 2: Jerome and Redmond send Neil´s men up|
|Renard takes over at Lille|
|Cancelo completes permanent Valencia move|
|Dnipro v Sevilla: Emery embracing chance at history|
|Kuchuk leaves Kuban Krasnodar|
|Desailly hails Terry and urges calm over Zouma|
|Guardiola has built on Heynckes´ Bayern legacy - Muller|
|Falcao thanks United fans|
|Zorniger takes Stuttgart role|
|Dani Alves to clarify Barca future after Champions League final|
|Pazzini in the dark over Milan future|
|Barzagli a doubt for Champions League final|
|Clough sacked by Sheffield United|
|Pardew eyeing ´quality´ signings|
|Carver encouraged by Ashley´s Newcastle promises|
|Gutierrez revels in Newcastle ´fairytale´|
|Relegation will haunt Hull - Chester|
|El Shaarawy eyes Italy call-up|
|Crouch delighted by headed goals record|
|Ancelotti all but confirms Real exit|
|Platini: FIFA will lack credibility if Blatter remains president|
|Bilbao duo pen new deals|
|Rooney: United can win title next season|
|Argentine football rocked by second death|
|Leicester must aim higher next season - Pearson|
|Monk negotiating new Swansea contract|
|Ox can play in cup final, says Wenger|
|Century of MLS goals ´surreal´ for Wondolowski|
|Copa Libertadores Preview: River not giving up hope|
|Cahill returns for Australia´s World Cup qualifier in Kyrgyzstan|
|MLS Review: Wondolowski joins 100-goal club|
|Toni on track for Hellas Verona´s maiden golden boot|
|Amid Ancelotti speculation, Galliani confirms Madrid trip|
|Berlusconi targets Ancelotti, Inzaghi remains committed to Milan|
|Serie A Review: El Shaarawy at the double in Milan victory, Fiorentina edge five-goal thriller|
|Taylor close to signing new Swansea contract|
|Milan 3 Torino 0: El Shaarawy grabs brace as Inzaghi´s men ease to victory|
|Bruce expresses sympathy with Hull owner and fans following relegation|
|Preston North End 4 Swindon Town 0: Beckford hat-trick seals promotion|
|Darmstadt promoted back to the Bundesliga after 33-year exile|
|Sherwood to assess Given injury ahead of FA Cup final|
|Europa League qualification would cap ´special´ season - Koeman|
|South Africa 0 Botswana 0 (6-7 on penalties): Hosts exit COSAFA Cup at first hurdle|
|Pearson hopeful of Cambiasso stay|
|Ings emotional after victorious ´last game´|
|Pochettino happy to finish fifth|
|West Brom release Mulumbu|
|Monk: Palace defeat not a reflection on season|
|LVG awaits news on ´minor´ Di Maria injury, refuses to comment on Falcao exit reports|
|Pulis accepts blame for West Brom´s Arsenal collapse|
|Toure and Milner were not saying goodbye - Pellegrini|
|Ramsey calls for patience in QPR rebuild|
|Allardyce: Time is right for West Ham exit|
|Mourinho already preparing for title defence|
|Eredivisie Review: Heerenveen to play Vitesse for Europa League place|
|Hughes hails Stoke showing|
|Carver still hoping to land Newcastle job|
|Sunderland squad needs better balance, says Advocaat|
|Pardew ´never dreamt´ of top-half finish at Palace|
|Survival Sunday: How the final-day Premier League relegation battle played out|
|Arsenal wanted Walcott stay regardless of hat-trick - Wenger|
|Bruce: Hull have not been good enough|
|De Gea has to say something - Van Gaal|
|Rodgers apologises after ´embarrassing´ defeat|
|Chelsea 3 Sunderland 1: Remy double secures win as Drogba bids farewell|
|Stoke City 6 Liverpool 1: Gerrard nets consolation in final-game thrashing|
|West Ham confirm Allardyce exit|
|Newcastle United 2 West Ham 0: Gutierrez on target as Carver´s men stay up|
|Crystal Palace 1 Swansea City 0: Chamakh earns season-closing win|
|Manchester City 2 Southampton 0: Lampard bids Premier League farewell with goal|
|Hull City 0 Manchester United 0: Bruce´s men relegated|
|Aston Villa 0 Burnley 1: Ings heads winner|
|Everton 0 Tottenham 1: Kane header seals fifth place|
|Leicester City 5 QPR 1: Vardy celebrates England call-up in style|
|Arsenal 4 West Brom 1: Wilshere screamer and trio for Theo secures third|
|Drogba carried from the pitch by Chelsea team-mates|
|Scottish Premiership Review: United take Dundee derby spoils, Celtic smash Inverness|
|Ashley not leaving Newcastle until club wins something|
|Klopp urges fans not to draw comparisons with Tuchel|
|Dortmund complete Castro capture|
|Stevens leaves Stuttgart after securing safety|
|Alves ruled out of Copa with serious knee injury|
|Drogba to leave Chelsea|
|Ashley is from another planet - Keegan|
|Sydney withdraw Janko offer amid talk of Toni|
|Cameron pens Stoke extension|
|Wimmer: I´m going to Tottenham|
|Returning Grabban ready for play-off final - Neil|
|Ferdinand ´overwhelmed´ by support following wife´s death|
|Odegaard enjoys ´nervous´ Real Madrid debut|
|Bamford fit but may be overlooked for play-off final|
|Barcelona´s best better than Real´s - Luis Enrique|
|Ancelotti laments key injuries in title failure|
|AFC Champions League Preview: Evergrande, Al Hilal desperate for wins|
|MLS Review: DC United deny nine-man Revolution, Rapids break drought|
|Bielsa hints at Marseille departure, congratulates Ligue 1|
|Silva, Matuidi eye fourth trophy|
|Jonas deserved golden boot - Jorge Jesus|
|Primeira Liga Review: Benfica celebrate title with home win|
|Ligue 1 Review: Monaco seal final Champions League spot, Marseille take fourth|